![]() ![]() This thesis focuses on optimizing the application of DNA as a bioreceptor for detection of L. Biosensors have been identified as a potential source of on-site monitoring for pathogenic threats. It is clear that this is a serious public health concern that could be better mitigated by improved forms of detection of the pathogen before human exposure. Reported cases of Legionnaires’ disease have increased dramatically since the turn of the century. Legionella pneumophila is one of the most notorious OPPPs and is presently one of the most threatening waterborne pathogens, particularly in developed countries. OPPPs exhibit thermal-tolerance, disinfectant-resistance, and growth under oligotrophic conditions, which make water distribution systems favorable habitats for their survival. One such threat has been identified as a group of waterborne pathogens known as opportunistic premise plumbing pathogens (OPPPs). After the Flint Water Crisis, there has been heightened public awareness on threats to potable drinking water, especially those induced by existing infrastructure. ![]()
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